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. 2016 Aug 17;8(1):248–267. doi: 10.18632/oncotarget.11335

Figure 5. TRAIL, QC and their combination treatment reduced the apoptosis in DR5 silenced MCF-10A-Tr cells.

Figure 5

Cells were treated as mentioned in methods material section and carried out the experiments. (A) i) Measurement of expression of DR5, DR4 and FADD by western blotting in scramble (SCR) siRNA (h) transfected cells.ii) Measurement of expression of DR5,DR4 and FADD by western blotting in DR5 Si RNA (h) treated cells. (B) Measurement of apoptosis by FACS analysis in DR5 silenced cells after the drug treatment. (C) Determination of apoptosis by annexin V-FITC/PI dual staining in DR5 silenced cells after the drug treatment. (D) Production of ROS in presence and absence of NAC in DR5 silenced cells. (E) Production of NO in presence and absence of L-NAME in DR5 knockout cells. Images shown were representative of three different experiments. Data are the mean ± SD of three independent experiments.