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. 2016 Nov 16;8(1):354–363. doi: 10.18632/oncotarget.13396

Figure 4. Effects of Ang-(1-7) on Spc-A1 cell EMT.

Figure 4

(A) Western blot analysis of E-Cadherin and Vimentin protein expression in Spc-A1 cells after treatment with PBS, 1μM A779 with 500 nM Ang-(1-7), 100 nM or 500 nM Ang-(1-7). Western blots are representative of three different experiments. (B) In vitro transwell migration assay. Non-transduced or Lenti-Ang-(1-7) transduced Spc-A1 cells were resuspended in serum free DMEM with or without 1.0 μmol/L the Ang- (1-7) receptor antagonist, A-779, and placed in the top portion of a transwell chamber. Migration ability of Spc-A1 cell was quantified by counting numbers of cells that adhere to the outside surface of the membranes. Five fields per well were counted. Values are expressed as mean ± SD of three independent experiments. *P < 0.05, vs.controls.