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. 2017 Mar 3;13(3):e1006263. doi: 10.1371/journal.ppat.1006263

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© 2017 Kahya et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — Qualitative (A) and quantitative (B) esterase activity assay using tributyrin (triglyceride) as substrate. (A) 1: EstA; 2: EstA^S121A; 3: Axe; 4: Axe^S181A; C: S. aureus lipase. 50 μg protein was added into each well. (B) The enzyme activity is expressed as micromoles of fatty acids released from the substrate per microgram of protein per minute. **p<0.01, ****: p<0.0001 compared to control (no enzyme). Data derived from 3 independent experiments.