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. 2017 Mar 3;13(3):e1006245. doi: 10.1371/journal.ppat.1006245

Fig 4. TEM analysis of SmD1 silenced cells.

Fig 4

Cells before and after silencing of SmD1 (40 hrs) were fixed, and ultra-thin sections were prepared and examined by TEM. The different ultra-structures are indicated. MVB, multivesicular bodies; ILV, intraluminal vesicles; M, mitochondrion; ER, Enodoplasmic reticulum; scale bar, 500 nm. (A and B) Un-induced cells (-Tet). (C-G) induced cells. Section showing MVB containing ILVs. (C-D) MVB located near the plasma membrane, and secreted exosome present on the cell surface. (E-F) EVs secreted from the cell membrane and flagella pocket. (G) Larger vesicles emerging from the flagellar membrane. The white arrow-heads indicate the ILVs and black arrow-heads the EVs.