Fig 10. Exosome secretion repels the migration of wild-type cells.
Cells (~107) were plated on semi solid agar containing tetracycline at a distance of 2.5 cm from each other. The SmD1 and SmD1/Vps36 silenced cells were plated on plates containing tetracycline. Pattern formation was analyzed 2 days after plating. The parasites from the plates were blotted, and the blot was stained with Ponceau and then reacted with anti-GPEET and anti-EP. The distance between the wild-type colony and the adjacent colony is presented. The statistical analysis represents the mean ± s.e.m **P< 0.01 comparing the distance between wild-type to wild-type, wild-type to SmD1and wild-type to SmD1/Vps36 silenced cells based on three independent experiments.