Skip to main content
. 2017 Mar 8;12:817–827. doi: 10.2147/COPD.S123405

Figure 4.

Figure 4

Cytokine profiles in COPD-induced WT and sγcTg BALF cells.

Notes: (A) Infiltrating cell numbers in BALF from WT and sγcTg mice exposed to CSE for 3 weeks. Data are presented as the mean and SEM of four WT and five sγcTg mice. (B) Infiltrating pattern of T or non-T cells in BALF from WT and sγcTg mice exposed to CSE for 3 weeks. Data are presented as the mean and SEM of four WT and five sγcTg mice. (C) BALF CD8 (top) or CD4 (bottom) T cells from CSE or PBS-treated WT and sγcTg mice were stimulated for 3 h with PMA and ionomycin and assessed for IFN-γ and IL-17A expression by intracellular staining. Dot blots are representative of four to five mice per group. (D) Bar graph shows percent (%) IFN-γ (left)- or IL-17A (right)-producing CD8 BALF T cells. Error bars represent the mean and SEM of four to five mice per group. (E) Bar graph shows percent (%) IFN-γ (left)- or IL-17A (right)-producing CD4 BALF T cells. Error bars represent the mean and SEM of four to five mice per group. *P<0.05, **P<0.01, and ***P<0.001.

Abbreviations: WT, wild type; sγcTg, soluble form of common gamma chain transgenic; BALF, bronchoalveolar lavage fluid; CSE, cigarette smoke extract; SEM, standard error of the mean; PBS, phosphate-buffered saline; NS, not significant; PMA, phorbol 12-myristate 13-acetate.