Multiplex PCR assay 1 of reference strains and clinical and stool samples. Lane 1, EPEC E2348/69 (eae and bfpA) and EAEC 042 (CVD432); lane 2, EPEC HSP43-1 (eae and bfpA); lane 3, atypical EPEC MA343-4 (eae); lane 4, EAEC MA233-1 (CVD432); lane 5, patient sample 5 (eae and bfpA); lane 6, patient sample 6 (eae); lane 7, patient sample 29 (CVD432); lane 8, stool sample 2 (eae and bfpA); lane 9, stool sample 8 (eae and bfpA); lane 10, stool sample 3 (CVD432); lane 11, stool sample 5 (CVD432); lane 12, stool sample 12 (CVD432); lane 13, stool sample 33 (eae); lane 14, stool sample 34 (eae); lane M, DNA molecular size markers (100-bp ladder).