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. 2016 Nov 19;8(1):512–522. doi: 10.18632/oncotarget.13473

Figure 4. Regulation of B cell function by hAT-MSCs.

Figure 4

(A) Human B lymphocytes were isolated from umbilical cord blood-derived mononuclear cells and isolation purity was determined by measuring the percentage of CD19-positive cells (B–G) B lymphocytes were co-cultured with two different donor-derived hAT-MSCs after induction of maturation. The proliferation of B lymphocytes was analyzed by (B) observing phase-contrast images and (C) counting the number of CD19-positive B lymphocytes. (D–E) Intensity of IgE expression among human CD19-positive cells was confirmed by flow cytometry with or without hAT-MSCs. (F–G) Mature B lymphocyte population was determined by measuring CD27- and CD19-double positive cells using flow cytometry. *P < 0.05, **P < 0.01, ***P < 0.001. Results are 1 representative experiment of 3 or the cumulative of 3 independent experiments. Results are shown as mean ± SD.