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. 2016 Nov 21;8(1):610–623. doi: 10.18632/oncotarget.13481

Figure 7. ROS was responsible for the SLNT-induced intrinsic apoptosis.

Figure 7

HT-29 cells were incubated with SLNT (800 μg/mL), an antioxidant NAC (5 μM), or combination for 48 h. Annexin V-FITC/PI staining was used to analyze cell apoptosis (A, B), ***p < 0.001 versus combination group, ###p < 0.001 versus NAC group. The expression of Bax, Bcl-2 and cytosol Cytochrome c were measured by western blot (C) and summarized (D). Each bar represents means ± SD (n = 3), *p < 0.05, **p < 0.01 versus combination group.