Figure 8. SLNT activated caspase-8, decreased nuclear NF-κB and increased TNF-α in vitro and in vivo.

Expression of cleaved caspase-8 and nuclear NF-κB in cells (A) and in tumors (B) were analyzed by western blot. Activity of caspase-8 in cells was further measured by Caspase-8 activity kit (C). Immunohistochemistry analysis of cleaved caspase-8 in NC and in SLNT (5.0 mg/kg) tumors was shown (D). The level of TNF-α in serum of nude mice (E) and in cell culture medium (F) was measured by ELISA assay. Each bar represents means ± SD, n = 3–6, ^*p < 0.05, ^***p < 0.001 versus NC group.