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. 2016 Nov 22;8(1):644–657. doi: 10.18632/oncotarget.13501

Figure 2. Abnormal expression of miR-506-3p alters the growth of NSCLC cells.

Figure 2

(A) Alarmar Blue assay showed that overexpression of miR-506-3p inhibits cell growth of A549 cells in 72 h, whereas inhibition of miR-506-3p in HCC827 cells promotes cell proliferation in 72 h. (B) Colony formation assay showed that colony ability of A549 cells was inhibited after treatment of miR-506-3p mimics in 6 days, while silencing of miR-506-3p in HCC827 cells promoted cell colony formation in 6 days. (C) FACS assay showed that overexpression of miR-506-3p promotes cell apoptosis of A549 cells in 48 h, whereas inhibition of miR-506-3p in HCC827 cells inhibits cell apotosis in 48 h. (D) Wound healing assay showed that cell mobility ability was inhibited when transfected by miR-506-3p mimics in A549 cells in 48 h, whereas silencing of miR-506-3p in HCC827 cells promotes cell mobility in 48 h. (E–F) Transwell assay showed that overexpression of miR-506-3p inhibits cell migration and invasion ability of A549 cells in 48 h, while inhibition of miR-506-3p in HCC827 cells promotes cell migration and invasion in 48 h. (G–H) The mean volume and weight of the xenograft tumors in miR-506-3p-A549 group was smaller than those of miR-NC-A549 group, whereas silencing of miR-506-3p in HCC827 cells promotes tumor weight than those of siRNA-miR-NC-HCC827 xenografted tumors. Asterisk (*) indicated P < 0.05.