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. 2016 Nov 23;8(1):705–721. doi: 10.18632/oncotarget.13525

Figure 11. miR-206 upregulates nuclear NF-κB p65 expression and TNF-α/IL-8/IL-1β levels in mouse colon.

Figure 11

(A) Western-blotting analysis of nuclear NF-κB p65 expression in mouse colon. Nuclear NF-κB p65 expression was higher in DSS-colitis mice than in control mice. miR-206 agomir and antagomir treatment led to increased and decreased nuclear NF-κB p65 expression in the colon of DSS-colitis mice, respectively, as compared with corresponding NC levels. (B) Representative western blotting showing NF-κB p65 expression in the indicated groups. ELISA analysis of the expression of TNF-α (C), IL-8 (D), and IL-1β (E). TNF-α, IL-8, and IL-1β levels were significantly increased in the colon of DSS-treated mice; moreover, TNF-α, IL-8, and IL-1β expression was significantly increased after miR-206-agomir treatment, but TNF-α and IL-8 expression was decreased following miR-206-antagomir treatment (relative to corresponding NC levels). All data are expressed as means ± SD. *P < 0.05 and **P < 0.01, compared with DSS-treated group; #P < 0.05 and ##P < 0.01, compared with corresponding NC group.