Skip to main content
. 2016 Nov 24;8(1):742–756. doi: 10.18632/oncotarget.13557

Figure 1. BCR stimulation with anti-IgM increases viability and enhances proliferation in primary CLL cells co-cultured with BMSC, CD40L and CpG ODN.

Figure 1

(A) Primary CLL cells were co-cultured with BMSC, CD40L and CpG ODN for 15 minutes and anti-IgM was added for 1 additional minute. Figure shows the immunoblot analysis of Akt and ERK1/2 phosphorylation from a representative patient. (B) Primary CLL cells were co-cultured with BMSC, CD40L, CpG ODN and anti-IgM for 24 and 48 hours. Viability was assessed in primary CLL cells from 9 patients by Annexin V and PI staining. (C) Mean % of Ki-67-positive cells from 9 patients was analyzed by FC. (*P < 0.05, ***P < 0.001, two-way ANOVA, Bonferroni's post-test. Graph shows mean ± SEM). PV: treatment with pervanadate.