Figure 3. TAK-659 induces higher degree of apoptosis than R406 in primary CLL cells.

(A) Primary CLL cells from 12 patients pre-treated with increasing doses of TAK-659 were cultured for 24 and 48 hours with BMSC, CD40L, CpG ODN and anti-IgM. Viability was assessed by Annexin V and PI staining by FC. Primary CLL cells from 26 patients pre-treated with increasing doses of TAK-659 were cultured for 48 hours in suspension, in co-culture, anti-IgM-stimulated or co-cultured and with anti-IgM stimulation (B); or pre-treated with increasing doses of TAK-659 or R406 in suspension (C), anti-IgM-stimulated (D), in co-culture (E) or co-cultured with anti-IgM-stimulation (F). LD₅₀ curves for TAK-659 and R406 are plotted on a logarithmic scale. LD₅₀ for every treatment cohort were calculated and compared (Two-way ANOVA, Bonferroni's post-test. Graph shows mean ± SEM).