Skip to main content
. 2016 Oct 19;8(1):757–768. doi: 10.18632/oncotarget.12748

Figure 6. ALOS4-CPT conjugate specifically induces DSBs and caspase 3 activation in WM-266-4 cells.

Figure 6

Western blot analyses with antibodies recognizing γH2A.X, active caspase 3 and histone H3 of lysates from cells treated with the indicated drugs for 3 hours and 24 hours. In each lane the band intensities of γH2A.X and active caspase 3 were quantified with ImageJ and normalized to the relative amount of histone H3. The graphs show the relative band intensities. The sample of the 3 hours DMSO treated cells was set as 1. A. WM-266-4 human metastatic melanoma B. non-malignant HEK293 cells.