Figure 6. Synergistic inhibition by dioscin and GCV of tumor growth in a mixed population of B16^tk cells and wild-type B16 cells in vivo.

Subcutaneous B16 tumors were established in C57BL/6J mice using B16^tk cells and wild-type B16 cells mixed at a ratio of 3:7. Mice were then divided into four treatment groups (n = 14 mice per group) : control (saline only), GCV only (100 mg/kg·day), dioscin only (50 mg/kg·day), and GCV plus dioscin. (A) Tumor volume was measured from day 11, once a day for 4 days. (B) Tumor weight was measured on day 14 following treatment. Data are presented as the mean ± standard error. *P < 0.05; **P < 0.01.