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. 2016 Nov 26;8(1):808–818. doi: 10.18632/oncotarget.13656

Figure 3. Cir-ZNF609 serves as a sponge for miR-150-5p.

Figure 3

(A) The levels of nuclear control transcript (U6), cytoplasmic control transcript (GAPDH), and cir-ZNF609 were assessed by qRT-PCR in nuclear and cytoplasmic fractions. (B) The expression of miR-150-5p in HSCR tissues and control tissues. MiR-150-5p was significantly rose in patient tissues compared with control tissues. (C) Sequence alignment of human miR-150-5p with cir-ZNF609. Bottom: mutations in the cir-ZNF609 sequence to create the mutant luciferase reporter constructs. (D) Luciferase reporter assay in 293T and SH-SY5Y cells after transfected with negative control or miR-150-5p mimics, renilla luciferase vector pRL-SV40 and the reporter constructs. Both firefly and renilla luciferase activities are measured in the same sample. Firefly luciferase signals were normalized with renilla luciferase signals. (E) RNA immunoprecipitation (RIP) experiments were performed using the Ago2 and IgG antibody to immunoprecipitate and primers were used to detect miR-150-5p and cir-ZNF609.