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. 2016 Nov 26;8(1):819–832. doi: 10.18632/oncotarget.13661

Figure 2. M2-like macrophages drive VM formation of glioma cells in vitro.

Figure 2

The glioma cells were incubated in the conditioned medium (CM) from THP-1 or M2 cells for 24 h. The glioma cells cultured in DMEM medium were used as control. (A, B) Representative images (A) and quantification (B) of tubule formation assay on Matrigel matrix in vitro (×100). (C) qRT-PCR was performed to evaluate the effect of THP-1-CM or M2-CM on VM markers. (D) Western blotting was used to detect the expression of VM associated proteins, including MMP-9, MMP-14 and LAMC2. Each bar represents the mean ± SEM (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001; ns, no significance.