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. 2016 Nov 26;8(1):819–832. doi: 10.18632/oncotarget.13661

Figure 3. M2-like macrophages amplify IL-6 expression in glioma cells.

Figure 3

(A) The Bio-Plex ProTM Human Th17 Cytokine Assay were used to detect the cytokines in the supernatant of THP-1, M2, U251, THP-1-CM/U251 coculture system and M2-CM/U251 coculture system. (B, C) Dose-dependent induction of IL-6 expression in human glioma cells by M2-CM for 24 h. The level of IL-6 mRNA was determined by qRT-PCR (B) and IL-6 concentration in the medium was detected using ELISA assay (C). (D) Time course of IL-6 and VM marker mRNA expression in glioma cells stimulated with M2-CM. Transcript levels were measured at indicated time after stimulation using qRT-PCR. Each bar represents the mean ± SEM (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001.