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. 2016 Dec 11;8(1):83–94. doi: 10.18632/oncotarget.13909

Figure 4. The chemokines and receptors expression in the thymus during mouse ontogeny.

Figure 4

A. The mRNA expression level of the CCR4 ligands CCL5, CCL17, and CCL22 and the CCR7 ligands CCL19 and CCL21 in whole thymi from Aire−/− mice and littermates were analyzed by qPCR. K-8 was used as internal standard. B. The mRNA expression of CCR4 and CCR7 in whole thymi. GAPDH was used as internal standard. The experiment was repeated for 4 times and a representative result is shown. C.The cell surface CCR7 expression in SP3 and SP4 thymocytes from 2- or 6-week old Aire−/− and littermate were shown in overlay histograms. D. 1μg CCL19 or PBS was intrathymically injected to the 2 weeks old Aire−/− RAG2p-GFP mice,littermate Aire+/+ RAG2p-GFP mice were administrated with PBS. 3 days later, CD4+ T cells were collected from the lymph nodes, and GFP fluorescence intensity was used to determine GFPhi and GFPlo RTEs cells. NS, no significance. * p < 0.05, ** p < 0.01.