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Western blotting was used to evaluate the protein expressions of ITGA6, FN1, CCND1, CCNE2 and TGFβR2 in PSMC2 silenced osteosarcoma cells and normal osteosarcoma cells. GAPDH and β-actin were used as a loading control. Histogram representing indicated the results of three independent experiments (**P < 0.01).
