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. 2004 Dec;42(12):5774–5782. doi: 10.1128/JCM.42.12.5774-5782.2004

FIG. 1.

FIG. 1.

Southern blot analysis of Cape Town M. tuberculosis isolates with ≤6 IS6110 insertions. Genomic DNA was restricted with PvuII and electrophoretically fractionated on agarose gels, and Southern hybridization was done with an enhanced-chemiluminescence-labeled IS-3′ probe. (A) IS6110 banding pattern of distinct principal genetic group 2 isolates from Cape Town, South Africa. The lanes are labeled according to the isolate names in Table 3. The band representing the IS6110 insertion in the DR region and bands representing IS6110 insertions in genes Rv0403c, Rv1758, Rv2787c, and Rv3018c are indicated. (B) IS6110 banding pattern of the principal genetic group 1 isolate from Cape Town, South Africa. The lane is labeled according to the isolate name given in Table 3. Cohybridization between the IS6110 and DR probes could not be demonstrated due to the presence of a PvuII site situated between the 3′ domain of the IS6110 element and the flanking DR region.