TABLE 1.
Conditions for multiplex PCRs of nine VNTR loci
| Multiplex | Conventional designationa | VNTR length (bp) | MgCl2 (mM) | PCR primer pairs (5′ to 3′, with labeling indicated) |
|---|---|---|---|---|
| Mix E | VNTR 2347 | 57 | 1.5 | GCCAGCCGCCGTGCATAAACCT (FAM) |
| AGCCACCCGGTGTGCCTTGTATGAC | ||||
| VNTR 2461 | 57 | ATGGCCACCCGATACCGCTTCAGT (VIC) | ||
| CGACGGGCCATCTTGGATCAGCTAC | ||||
| VNTR 3171 | 54 | GGTGCGCACCTGCTCCAGATAA (NED) | ||
| GGCTCTCATTGCTGGAGGGTTGTAC | ||||
| Mix F | VNTR 0424 | 51 | 1.5 | CTTGGCCGGCATCAAGCGCATTATT |
| GGCAGCAGAGCCCGGGATTCTTC (FAM) | ||||
| VNTR 0577 | 58 | CGAGAGTGGCAGTGGCGGTTATCT (VIC) | ||
| AATGACTTGAACGCGCAAATTGTGA | ||||
| VNTR 1895 | 57 | GTGAGCAGGCCCAGCAGACT (NED) | ||
| CCACGAAATGTTCAAACACCTCAAT | ||||
| Mix G | VNTR 2401 | 58 | 3.0 | CTTGAAGCCCCGGTCTCATCTGT (FAM) |
| ACTTGAACCCCACGCCCATTAGTA | ||||
| VNTR 3690 | 58 | CGGTGGAGGCGATGAACGTCTTC (VIC) | ||
| TAGAGCGGCACGGGGGAAAGCTTAG | ||||
| VNTR 4156 | 59 | TGACCACGGATTGCTCTAGT | ||
| GCCGGCGTCCATGTT (NED) |