TABLE 2.
Primers used for amplification
| Target(s)a | Primer | Sequenceb | Product size (bp) | Annealing temp (°C) | Nucleotide positions (bp)c | GenBank accession no.d |
|---|---|---|---|---|---|---|
| CTX-M group I | CTXM1-F3 | GAC GAT GTC ACT GGC TGA GC | 499 | 55 | 416-435 | X92506 |
| CTXM1-R2 | AGC CG C CGA CGC TAA TAC A | 914-896 | ||||
| CTX-M group II | TOHO1-2F | GCG ACC TGG TTA ACT ACA ATC C | 351 | 55 | 313-334 | X92507 |
| TOHO1-1R | CGG TAG TAT TGC CCT TAA GCC | 663-643 | ||||
| CTX-M group III | CTXM825F | CGC TTT GCC ATG TGC AGC ACC | 307 | 55 | 475-495 | AF189721 |
| CTXM825R | GCT CAG TAC GAT CGA GCC | 781-764 | ||||
| CTX-M group IV | CTXM914F | GCT GGA GAA AAG CAG CGG AG | 474 | 62 | 1857-1876 | AF252622 |
| CTXM914R | GTA AGC TGA CGC AAC GTC TG | 2330-2311 |
a
Group I includes CTX-M-1, -3, -10 to -12, -15 (UOE-1), -22, -23, -28, -29, and -30. Group II includes CTX-M-2, -4 to -7, and -20 and Toho-1. Group III includes CTX-M-8. Group IV includes CTX-M-9, -13, -14, -16 to -19 and -21, and -27 and Toho-2. Finally, group V includes CTX-M-25 and -26.
b
Sequence of primer as synthesized 5′ to 3′.
c
Nucleotide position in base pairs for the GenBank accession number sequence.
d
Accession number of the sequence used for primer design.