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. 2016 Dec 21;8(5):7391–7404. doi: 10.18632/oncotarget.14080

Figure 5. Swimming decreases the expression of NF-κB p65 and COX2 protein in the colon of rats with DSS-induced colitis.

Figure 5

After 7 weeks of swimming exercise, colon tissues were collected and used for immunohistochemical investigation, or were homogenized in tissue lysis buffer prior to Western blot analysis. A. Representative images of IHC-stained tissues showing NF-κB p65 and COX2 expression (100× magnification). B. Immunoreactive scores of the NF-κB p65 and COX2 shown in A.. C. Representative blots showing COX2 and p-NF-κB p65 expressions after Western blot analysis. β-actin and lamin B were used as endogenous loading controls. D. The COX2/β-actin and p-NF-κB p65/lamin B ratios. Data are representative data of three independent experiments and are expressed as the mean ± SD. *P < 0.05 vs. Ctrl groups; #P < 0.05 vs. DSS + Sed group.