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. 2016 Dec 21;8(5):7391–7404. doi: 10.18632/oncotarget.14080

Figure 6. Swimming ameliorates oxidative stress and increases antioxidant defense in the colon of rats with DSS-induced colitis.

Figure 6

After 7 weeks of swimming exercise, colon sections were collected and homogenized in tissue lysis buffer and centrifuged, and the supernatants were harvested. A. ROS production was measured in terms of relative DCF fluorescence. B. Lipid peroxide was measured in terms of MDA production. C. iNOS activity was measured in a standard assay that monitors the conversion of arginine to citrulline. D. Nitrite production was assessed in a colorimetric reaction with the Griess reagent. E. GSH levels were determined using DTNB reagent. F. TAC was determined using a commercial antioxidant assay kit. G. SOD activity was assessed according to inhibition of pyrogallol auto-oxidation. H. GPx activity was determined according to its ability to oxidize GSH. Data are representative of three independent experiments and are expressed as the mean ± SD. *P < 0.05 vs. Ctrl groups; #P < 0.05 vs. DSS + Sed group.