Figure 3. EMT and CSCs related genes differentially expressed in Very Responsive, Responsive and Low Responsive PDXs.

EMT (panel A) and CSC-related genes (panel B) found to be predictive of cDDP response. Real-time PCR data were pre-processed using the geometric mean of the available and appropriate housekeeping genes as endogenous control (ACTB, B2M and HPRT1 for EMT plates; ACTB, B2M, GAPDH, HPRT1 and RPLP0 for CSCs plates). Data were expressed as arbitrary base-2 logarithmic units (2-deltaCt) in Very Responsive (□, VR), Responsive (■, R) and Low Responsive (■, LR) xenografts. EMT (panel C) and CSCs genes (panel D) validations by real-time PCR using custom-designed primers. Data are expressed as gene expression values normalized by the expression of housekeeping genes as specified in Materials and Methods. *p < 0.05, **p < 0.005, ***p < 0.0005.