Figure 2. SHP2-deficiency leads to decreased development of colitis-associated colon (CAC) cancer.

WT and SHP2^CD4−/− mice were subjected to AOM-based induction protocol using three cycles of 2.5% DSS in the drinking water. (A) The colon was photographed. (B) The numbers, sizes and load of the tumors were measured. (C–E) H&E-staining in (C), IHC analysis of PCNA expression in (D), and immunoblotting of PCNA expression in (E) using colonic tissues from CAC mice on day 80. Data are representative of three independent experiments (mean ± SEM of 12 mice per group). Scale bar, 100 μm. *P < 0.05, **P < 0.01 vs. WT group.