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. 2016 Dec 7;8(5):7586–7597. doi: 10.18632/oncotarget.13812

Figure 5. IFN-γ neutralization reverses the inhibitory effect of SHP2-deficiency on CAC.

Figure 5

Mice were injected intraperitoneally with 25 μg anti-IFN-γ neutralized antibody or IgG control every 4 days after the last DSS cycle. (A) The inside of the colon was photographed. (B) The numbers, sizes and load of the tumors were measured. (C) Real-time PCR analysis of perforin 1, granzyme B, and FasL expression in colonic tissues from CAC mice on day 80. (D) Flow cytometry analysis of Annexin V+ YAC-1 cells in the co-culture with CD8+ cytotoxic T lymphocyte isolated from CAC mice at day 80. Target (T) cells: YAC-1 ; Effector (E) cells: CD8+ T cells. *P < 0.05 vs. IgG group. (E) Immunofluorescence analysis of CD8+ T cell infiltration in colonic tissues from CAC mice on day 80. Scale bar, 100 μm. (F) Immunoblotting analysis of PCNA expression on colonic tissues from CAC mice on day 80. Data are representative of three independent experiments (mean ± SEM of 6 mice per group). *P < 0.05, **P < 0.01.