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. 2016 Dec 7;8(5):7598–7613. doi: 10.18632/oncotarget.13814

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Copyright: © 2017 Vázquez et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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(A) Cells were seeded under normoxic and hypoxic conditions and treated with OTX015 for 72 h. The antiproliferative effect was determined by cell counting. The diagonal-line pattern () indicates OTX015 concentrations that decreased cell growth by 50% (GI₅₀). For each treatment, the influence of hypoxia on antiproliferative activity was evaluated with the Student t-test. Significant differences in the OTX015 treatments were determined with a one-way ANOVA test (p < 0.01) followed by an SNK a posteriori test; substantial differences (p < 0.05) are indicated as letters above the bars. (B) Cell lines were treated with OTX015 for 72 h (indicated by the green area) and then cultured in drug-free medium. The Y axis represents the number of cells per well evaluated every 24 h after cell seeding (time 0). Significant differences in the number of control and treated cells at each time point were determined by two-way ANOVA test (p < 0.001) followed by a Bonferroni a posteriori test (*p < 0.05, **p < 0.01, ***p < 0.001). Significant differences at each time point after washout versus 72 h-treated cells were determined with one-way ANOVA test (p < 0.01) followed by an SNK a posteriori test (^#p < 0.05). (C) Cells were treated with OTX015 for 24, 48 and 72 h and the latter cells were further cultured without OTX015 for 48 h (drug washout). Significant differences in the percentage of OTX015-treated cells in the G1, S and G2/M phases with respect to untreated controls were evaluated by a one-way ANOVA test (p < 0.01) followed by an SNK a posteriori test. After washout, differences in the percentage of cells in a given cell cycle phase between control and pretreated cells were determined with the Student t-test. Significant differences between treated cells and controls were reported for the G1 phase (*p < 0.05, **p < 0.01), S phase (^#p < 0.05, ^##p < 0.01) and G2/M phase (×p < 0.05, ××p < 0.01). Each dot or bar and vertical line represents the mean ± SEM, respectively (n ≥ 3).

Inline graphic — (A) Cells were seeded under normoxic and hypoxic conditions and treated with OTX015 for 72 h. The antiproliferative effect was determined by cell counting. The diagonal-line pattern () indicates OTX015 concentrations that decreased cell growth by 50% (GI₅₀). For each treatment, the influence of hypoxia on antiproliferative activity was evaluated with the Student t-test. Significant differences in the OTX015 treatments were determined with a one-way ANOVA test (p < 0.01) followed by an SNK a posteriori test; substantial differences (p < 0.05) are indicated as letters above the bars. (B) Cell lines were treated with OTX015 for 72 h (indicated by the green area) and then cultured in drug-free medium. The Y axis represents the number of cells per well evaluated every 24 h after cell seeding (time 0). Significant differences in the number of control and treated cells at each time point were determined by two-way ANOVA test (p < 0.001) followed by a Bonferroni a posteriori test (*p < 0.05, **p < 0.01, ***p < 0.001). Significant differences at each time point after washout versus 72 h-treated cells were determined with one-way ANOVA test (p < 0.01) followed by an SNK a posteriori test (^#p < 0.05). (C) Cells were treated with OTX015 for 24, 48 and 72 h and the latter cells were further cultured without OTX015 for 48 h (drug washout). Significant differences in the percentage of OTX015-treated cells in the G1, S and G2/M phases with respect to untreated controls were evaluated by a one-way ANOVA test (p < 0.01) followed by an SNK a posteriori test. After washout, differences in the percentage of cells in a given cell cycle phase between control and pretreated cells were determined with the Student t-test. Significant differences between treated cells and controls were reported for the G1 phase (*p < 0.05, **p < 0.01), S phase (^#p < 0.05, ^##p < 0.01) and G2/M phase (×p < 0.05, ××p < 0.01). Each dot or bar and vertical line represents the mean ± SEM, respectively (n ≥ 3).