TABLE 3.
Sequence primers
| Primer | Sequence (5′→3′) | Positiona |
|---|---|---|
| rBseq1 | ATG TCC TTG ATG GAC TTC GCA | −374 |
| rBseq2 | CAA GAC TGT CAC CTA TAC CCG T | +699 |
| rBseq3 | ATA TCG GTG CGT ATA TCC GCA | +1055 |
| rBseq4 | CGT ATC GCA AGG TTG TCG AT | +1799 |
| rBseq5 | GCA TGG AAC CGA TCG TGG | +2138 |
| rBseq6 | CGA CGT GTT CAC CTC GAT TC | +2502 |
| rBseq7 | GAA ATC GAG CGT CTG GCC AA | +2881 |
| rBseq8 | CTG TGA AGC GCA AGA TCC A | +3230 |
| rBseq9 | ATG CTG GCC AAC CAG GTG AA | +3577 |
a
The numbering position is based on the submitted rpoB gene sequence of B. melitensis 16M (accession number AE009516).