TABLE 4.
Mutations identified in analyzing the rpoB gene in Rifr Brucella strainsa
| Rifr strain | Missense mutation
|
Rifs parental strain | ||
|---|---|---|---|---|
| Amino acid(s) affected | Codon alteration(s) | Amino acid change(s) | ||
| B. abortus RB51 | 526 | GAC→TAC | Asp→Tyr | B. abortus 2308 |
| B. melitensis mutants | ||||
| CT-6, -7, -10, -14, and -20 | 154 | GTT→TTT | Val→Phe | B. melitensis CT1p |
| CT-2 and -11 | 526 | GAC→TAC | Asp→Tyr | B. melitensis CT1p |
| CT-15 | 526, 539 | GAC→AAC, CGC→AGC | Asp→Asn, Arg→Ser | B. melitensis CT1p |
| CT-19 | 526, 574 | GAC→GGC, CCG→CTG | Asp→Gly, Pro→Leu | B. melitensis CT1p |
| CT-9 | 526, 574 | GAC→AAC, CCG→CTG | Asp→Asn, Pro→Leu | B. melitensis CT1p |
| CT-3 and -18 | 536 | CAC→CTC | His→Leu | B. melitensis CT1p |
| CT-1, -4, -5, -8, -12, -13, and -16 | 536 | CAC→TAC | His→Tyr | B. melitensis CT1p |
| CT-17 | 541 | TCG→TTG | Ser→Leu | B. melitensis CT1p |
a
The rpoB codon numbering is based on the B. melitensis 16M rpoB numbering system (accession no. AE009516). Both the nucleotide and the amino acid changes were identified by comparison with the corresponding sequences of the Rifs parental Brucella strains.