Figure 3. Bcl-2 and Twist1 form a complex in OSCC.

(A) Coimmunoprecipitation was used to determine the interaction of two proteins. Hypoxia treatment was performed in normal Tca8113 cells for 12 hours and Tb3.1 cells for 24 hours. Positive staining could be found in either normal conditions or cell precipitation samples treated with hypoxia in the two cell lines. Hypoxia could boost its interaction. (B) and (C) Expression of Bcl-2 and Twist1 in the nucleus and cytoplasm following transfection of shRNA against Bcl-2 and Twist1 alone or together in the presence of hypoxia. Immunofluorescence staining examined the dynamic colocalization of these two proteins within single living cells. The Bcl-2 expression is shown in red, whereas Twist1 expression is shown in green. The cells were cultured in hypoxia conditions.