Table 2. Expression of Bcl-2, Twist1 and EMT related proteins were determined by immunohistochemistry, and clinic-pathologic variables in 82 OSCC.

Variables		Twist1				Bcl-2				E-cadherin				N-cadherin				Vimentin				MMP2				MMP9
	Total	High	Low	χ2	P	High	Low	χ2	P	High	Low	χ2	P	High	Low	χ2	P	High	Low	χ2	P	High	Low	χ2	P	High	Low	χ2	P
Gender
Male	56	29	27	2.075	0.150	30	26	1.794	0.180	28	28	0.421	0.636	32	24	1.090	0.339	36	20	0.058	0.811	34	22	2.075	0.150	42	14	1.958	0.162
Female	26	17	9			18	8			15	11			18	8			16	10			20	6			23	13
Age
≤60	49	29	20	0.363	0.547	30	19	0.362	0.547	24	25	0.584	0.503	32	17	0.960	0.363	31	18	0.001	1.000	31	18	0.363	0.547	39	10	0.008	0.930
>60	33	17	16			18	15			19	14			18	15			23	10			23	10			26	7
T stage
T1 & T2	47	24	23	0.844	0.358	26	21	0.470	0.493	24	23	0.083	0.826	28	19	0.091	0.363	29	18	0.139	0.818	29	18	0.844	0.358	34	13	3.216	0.073
T3 & T4	35	22	13			22	13			19	16			22	13			23	12			25	10			31	4
Lymphnode metastasis
Yes	40	29	11	6.950	0.008	29	11	4.228	0.040	16	24	4.845	0.046	29	11	4.359	0.044	31	9	6.678	0.012	32	8	6.950	0.008	37	3	8.320	0.004
No	42	17	25			19	23			27	15			21	21			21	21			22	20			28	14
Histology grade
Grade 1	42	18	24	4.711	0.030	20	22	6.274	0.012	28	14	6.988	0.014	20	22	6.455	0.014	22	20	4.518	0.041	23	19	4.711	0.030	29	13	5.473	0.019
Grade 2 & 3	40	28	12			28	12			15	25			30	10			30	10			31	9			36	4
Smoking & Drinking
Yes	38	22	16	0.000	0.991	23	15	0.116	0.743	22	16	0.845	0.384	22	16	0.282	0.654	27	11	1.781	0.251	25	13	0.000	0.991	28	10	1.344	0.246
No	44	24	20			25	19			21	23			28	16			25	19			29	15			37	7