Figure 3. Effect of BEZ235, BKM120 and everolimus (EVE) on LMS cell apoptosis.

Representative dot-plot diagrams of flow cytometry with annexin V/PI for LMS cells treated for 72 hours with BEZ235, BKM120 and EVE at 10-fold IC₅₀ values (A). Percentage of apoptotic cells after treatment for 72 hours with 3 drug concentrations (1/10 IC₅₀, IC₅₀ value, 10-fold IC₅₀) (B). Data are presented as the mean ± SEM of three independent experiments. *, drug p < 0.05 vs. control; ***, drug p < 0.001 vs. control (two-way ANOVA).