Table 1. TSY-1 induced changes of expression in telomere associated genes in HL-60, PBMCs and HSCs.

Telomere Maintenance				Telomere Associated Complex				Telomere Regulation				Other Telomerase-associated Genes
Gene Target	HL60	PBMCs	HSCs	Gene Target	HL60	PBMCs	HSCs	Gene Target	HL60	PBMCs	HSCs	Gene Target	HL60	PBMCs	HSCs
DCLRE1C	−1.37	1.15	−1.33	RAP1A	1.11	−1.10	1.10	TGFB1	1.32	−1.08	1.12	ATM	−1.28	1.14	1.34
HSPA1L	4.52	5.74	2.85	ACD	−1.67	1.55	1.49	SSB	−1.76	1.04	1.17	CDK2	−2.59	1.06	1.00
MRE11A	−2.14	1.14	1.08	POT1	1.01	1.38	−1.32	SP1	1.07	1.05	−1.06	CHEK1	−1.73	1.83	−1.48
NBN	1.01	1..59	−1.05	TINF2	1.08	−1.01	−1.73	SART1	−1.24	1.02	1.37	CHEK2	−2.10	1.06	−1.12
PARP1	−2.22	1.10	−1.28	TERF1	−1.02	−1.57	−1.26	RB1	1.14	1.03	1.39	HAT1	−1.36	1.34	1.20
PIF1	1.36	1.69	2.05	TERF2	−1.13	1.11	1.08	RAPGEF1	1.25	−1.01	1.59	HNRNPA2B1	−2.08	1.11	1.03
PRKDC	−2.27	−1.15	−1.46	GAR1	−1.96	1.20	−1.47	RASSF1	1.96	1.23	1.05	HNRNPD	−1.47	1.21	1.08
PTGES3	−1.55	1.75	2.48	NHP2	−2.21	−1.21	−1.37	TP53	-	1.04	−1.14	HSP90AA1	−1.30	2.34	2.61
RAD50	−1.23	−1.12	1.05	NOP10	1.01	1.55	1.14	DCLRE1B	−1.76	1.01	−1.13	NCL	−2.08	1.05	−1.26
RFC1	−1.12	−1.05	−1.44	TERT	−5.55	1.69	8.12	KRIT1	−1.37	−1.07	−1.08	OBFC1	1.11	−1.60	−2.00
SMG6	1.11	−1.07	1.24	DKC1	−1.24	−1.17	−1.24	MYC	−4.58	−1.40	−1.32	PURA	1.64	1.31	2.35
TNKS2	−1.23	1.13	−1.06	EME1	−1.07	−1.89	1.13	SMAD3	1.25	1.69	8.12	RAD17	−1.13	1.05	1.19
RTEL1	−2.34	1.05	1.22	MSH2	−1.57	−1.17	−1.35	ABL1	−1.27	1.18	−1.24	RIF1	−1.30	1.11	−1.56
TEP1	1.08	1.50	1.29	MSH3	−1.52	1.07	−1.22	AKT1	−1.65	1.12	−1.08	SIRT2	−1.53	1.19	1.24
TNKS	1.03	−1.14	−1.15	MUS81	1.26	1.05	1.21	ATP5C1	−1.37	1.08	1.17	SIRT6	−1.34	1.13	1.38
POT1	1.01	1.38	−1.32	PLK1	−1.12	−1.75	−1.40	BCL2	−1.84	1.61	−1.28	SUN1	1.49	1.34	2.55
TINF2	1.08	−1.01	−1.73	SLX4	−1.06	−1.10	1.04	EGF	1.67	-	2.32	TP53BP1	−1.86	−1.08	−1.17
TERF1	−1.02	−1.57	−1.26	ERCC1	1.05	1.28	1.19	IGF1	-	-	4.51
ERCC4	−1.05	1.05	1.16	ERCC4	−1.05	1.05	1.16	KRAS	−1.30	1.20	−1.07
TERF2	−1.13	1.11	1.08	TERF2	−1.13	1.11	1.08	MEN1	−1.99	1.02	−1.20
TERF2IP	−1.02	1.01	1.10	TERF2IP	−1.02	1.01	1.10	PAX8	−1.24	−1.26	2.48
DKC1	−1.24	−1.17	−1.24					PINX1	−1.52	1.09	−1.03
TERT*	−5.55	1.69	8.12					PPARG	7.30	−3.43	3.98
MYC	−4.58	−1.40	−1.32					PPP2R1A	−1.20	1.28	1.20
ERCC1	1.05	1.28	1.19					PPP2R1B	−1.42	1.41	−1.32
ACD	−1.67	1.55	1.49					PRKCA	1.95	−1.43	1.91
BLM	−1.97	1.17	1.13					PRKCB	1.93	1.00	1.21

The gene target that shows the most prominent differential effect between HL-60 (down regulation) and PBMCs / HSCs (up regulation) is TERT gene. Others (ACD, BLM, ATP5C1, BLM, CDK2, ERCC4, HAT1, HNRNPD, HSP90A1, MRE11A, PPP2R1A, PTGES3, RAD17, RTEL1, SART1, and SSB), have the same direction, but with minimal effect (mostly <2-fold change). PBMC: normal peripheral blood mononuclear cells, HSCs: CD34+ hematopoietic stem cells.