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. 2016 Dec 24;8(5):8173–8188. doi: 10.18632/oncotarget.14132

Figure 5. Nutrient dependence of AldeRed fluorescence in N18TG2 cells.

Figure 5

A. Typical fluorescence images of cells stained for 30 min with AldeRed after 2 h 30 min incubation in 25 mM glucose (left) or 3.85 mM glutamine (right) medium. Scale bar is 20 μm. B. Comparison of the relative AldeRed fluorescence changes in cells incubated in glucose (Glc) or glutamine (Gln) medium. Measured ALDH fluorescence was normalized to ALDH fluorescence of cells incubated in glucose medium. Data are presented as mean values ± SEM for 2 independent experiments, (>600 cells analyzed). * p < 0.05.