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. 2016 Dec 24;8(5):8173–8188. doi: 10.18632/oncotarget.14132

Figure 6. Morphology, viability and nucleus morphology of cells incubated in different media after addition of HNE observed with brightfield and fluorescent microscopy.

Figure 6

Cells were incubated for 150 min in medium containing either A., E. and I. 25 mM glucose, or B., F. and H. 3.85 mM glutamine medium, or C. full medium, or D. 24.5 mM glucose and 3.85 mM glutamine. The concentration of HNE added for 1 h was 16 μM. A-D. Morphology, E and F. Reversibility of the mitochondrial membrane potential: cells were washed after HNE treatment and incubated for 3 hours in fresh medium with TMRE. G. Viability: The percentage of propidium iodide (PI) positive cells and caspase 3 activity was determined after 1 h incubation with HNE. Data are presented as mean values ± SEM for 5 independent experiments. * p < 0.05. H and I. Cell nuclei were stained with DAPI. Scale bar is 10 μm.