Skip to main content
. 2016 Dec 24;8(5):8283–8293. doi: 10.18632/oncotarget.14164

Figure 1. Down regulation of ASIC1a in NS20Y cells reduced CPT-cAMP-induced neurite growth.

Figure 1

NS20Y cells were transfected with a short hairpin ASIC1a (sh ASIC1a) or a control vector tagged with GFP, cells were left untreated or treated with 1 mM CPT-cAMP for 72h. After fixation the cells were probed with antibodies as indicated and photographed at 40x using fluorescecnt microscope. A. The parental NS20Y cells are round and spindle shaped, there is no dendrite on the cell body. After treated with 1 mM CPT-cAMP, cells showed polygonal shaped cells with dendrites all over the cell body. All cells were MAP2 positive by double immunostaining, implying that they were neuronal origin. B. Quantitative measurement of neurite lengths using Simple Neurite Tracer software (upper panel) showed that average neurite length was 2.6-fold of control in cells treated with CPT-cAMP (n=34), while this increase was reduced by shASIC1a to only 1.4-fold of control (n=20) (lower panel).