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. 2016 Dec 24;8(5):8283–8293. doi: 10.18632/oncotarget.14164

Figure 3. Down regulation of ASIC1a by shRNA increases while up regulation of ASIC1a decreases Notch1 and its target gene Survivin in NS20Y cells.

Figure 3

The shASIC1a and pEGFP-ASIC1a constructs were transfected into NS20Y cells and the changes in expression levels of Notch1signaling were then determined by Western blot. A. The levels of endogenous expression of Notch1 and its downstream targeting gene, Survivin were detected in NS20Y cells. Both Notch1and Survivin are constitutively expressed in NS20Y (lane 1 and 5 in upper panel), knock-down of shASIC1a resulted in upregulation of Notch1 gene and its downstream target Survivin gene (upper left panel, lane 1 vs. 2 and lane 3 vs.4); B. While overexpression of ASIC1a downregulated Notch1 and Survivin genes (upper right panel).