Figure 5. DAPT, an inhibitor of Notch1 significantly abolishes inhibition of neurite extension in NS20Y cells.

To test whether the ASIC1a and Notch molecules act synergistically or dependently upon neuronal differentiation, we inactivated Notch signaling by DAPT, a commonly used Notch inhibitor, and then tested the effect of ASIC1a signaling on neuronal differentiation and neurite growth. NS20Y cells were transfected with shASIC1a for 24h, and treated with DAPT (50 nM) for 48h. Morphologically, average neurite length was increased after the cells were treated with DAPT in different groups: A. ctrl vs DAPT, CPT-cAMP vs. CPT-cAMP+DAPT; B. shASIC1 vs shASIC1+DAPT, shASIC1+CPT-cAMP vs shASIC1+CPT-cAMP+DAPT