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. 2016 Dec 26;8(5):8369–8382. doi: 10.18632/oncotarget.14207

Figure 4. YM155 induces apoptotic cell death in GEP-NEC cell lines.

Figure 4

Annexin-V/PI staining and FACS analysis of NEC-DUE1 A. or -DUE2 B. cells revealed a dose dependent increase of annexin positive apoptotic cells when incubated with YM155 for 48 hours. Increased and dose dependent Caspase-3/7 activity was observed in NEC-DUE1 C. and -DUE2 D. cells when treated with YM155 for 2 hours. E, PARP cleavage became obvious in the more sensitive NEC-DUE2 cell line. Cells were treated at indicated YM155 concentrations for 24 hours. Protein lysates were separated by SDS-PAGE and blotted membranes detected with specific antibodies recognizing uncleaved as well as cleaved PARP (cPARP). Statistical significance was calculated by two-tailed nonparametric Mann-Whitney test (**indicates a p-value ≤0.01; *indicates a p-value ≤0.05).