Skip to main content
. 2016 Dec 27;8(5):8436–8446. doi: 10.18632/oncotarget.14246

Figure 5. MRTF-A effected the Nrf2 expression through binding to the CarG box of Nrf2 promoter.

Figure 5

The promoter region of Nrf2 was inserted into pGL-3(Nrf2-luc), and point mutation of Nrf2 promoter region was constructed by circular PCR(mut-Nrf2-luc). For transfection experiments, the Cos-7 cells were transfected with 0.8 μg MRTF-A and 0.2 μg Nrf2-luc or mut-Nrf2-luc. Luciferase assay was performed (*, P<0.05, n=3) A. and B. The vector pcDNA3.1 alone was used as a negative control. C. Chromatin immunoprecipitation (ChIP) assay was used to determine whether MRTF-A can change Nrf2 transcriptional activity through binding on CarG box.