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. 2015 Dec 19;8(5):8801–8806. doi: 10.18632/oncotarget.6688

Figure 1. Detection of CTCs.

Figure 1

Cells from healthy donor blood and CTC revealed by subtraction enrichment combined with iFISH are shown (right). Cells from healthy donor blood were positive for CD45 and negative for CEP8 (upper-right). Arrow indicates aneuploidy CTC identified as DAPI+/CEP8+/CD45- (lower-right). Negative control and isotype control are shown (left). “None” indicates cells without any antibody staining or probe hybridizing, “CD45” indicates isotype control of CD45 antibody (mouse IgG2a), “FISH” indicates cells performed FISH without hybridization probe added, and “Both” indicates cells performed iFISH with isotype of CD45 antibody staining and no probe hybridizing.