Table 1.

DEVDase activity associated with photodamage

Conditions	DEVDase activity
Control	0.07 ± 0.01
BPD alone	0.21 ± 0.04
NPe6 alone	0.11 ± 0.03
BPD → NPe6 sequential irradiation, no gap	2.6 ± 0.15*
BPD → NPe6 | 3' gap	2.3 ± 0.11*
BPD → NPe6 | 10' gap	1.8 ±0.15*
BPD → NPe6 | 30' gap	0.9 ± 0.10
BPD → NPe6 | 30‘ gap (cells held at 15°C)	1.4 ± 0.18*
BPD/NPe6 | simultaneous irradiation	2.8 ± 0.14*

1c1c7 cultures were treated with 20 µM NPe6 and 0.5 µM BPD for 1 h, before irradiation as indicated in the text. DEVDase activity was measured 2 h after irradiation.

DEVDase units = nmol product/min/mg protein.

Values represent mean ± SD for three determinations.

^*Statistically different from control values (P < 0.05).