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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: J Neurosci Res. 2016 Nov 8;95(5):1144–1160. doi: 10.1002/jnr.23970

Figure 2. Generating CNVs using CRISPR.

Figure 2

A. To generate an inversion or deletion, CRISPRs are designed at the desired breakpoints to create DSBs. The resulting inversion or deletion is generated only if the DSBs are created on the same homologous chromosome (cis).

B. If the DSBs are generated on different homologous chromosomes (trans), this may result in a duplication.

Generation of DSBs in cis or trans is random and cannot be controlled.

C. As an example of how to generate a translocation, two CRISPRs are needed. The first CRISPR is designed to create a DSB outside the first gene of interest on chromosome 3, and the second CRISPR is designed to create a DSB outside the other gene of interest on chromosome 10.