Figure 3. Generating subdeletions using CRISPR.

Designing subdeletions of a CNV is relatively simple. Briefly, the recurrent deletion is divided into three (or more) segments (A, B, C). Each of these segments is separately deleted using the corresponding CRISPR. Functional studies can then be done on each subdeletion to determine which segment contains the gene(s) that confer risk for a disorder, such as schizophrenia (SZ).

Other rearrangements are possible with this design, as outlined in Figure 2.