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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Allergy. 2016 Nov 11;72(4):656–664. doi: 10.1111/all.13067

Figure 2. CHS-dependent dermal vascularization is inhibited by OA-NO2.

Figure 2

DNFB CHS responses were initiated following treatment with SC OA-NO2. (a) 72 h and 144 h following elicitation ears were excised and cross-sections were immunofluorescently labeled with CD31-specific antibodies (red) in addition to DAPI nuclear counterstain (blue). Dashed line indicates epidermal-dermal junction. Scale bar = 50 μm. (b) CD31 expression was quantitated by assessing the red pixel density. Bars represent the mean density of 10 independent high powered fields ± SD. Asterisks indicate a significant difference compared to DNFB alone at each time point. *** = p < 0.001 and * = p < 0.05.