Figure 13. 2D western blot analyses with extracts from adult zebrafish Heart and skeletal muscle.

1. The Coomassie stained zebrafish skeletal muscle protein across the gel.
2. The PVDF filter was stained with CH1 monoclonal antibody followed by treatment with secondary antibody as mentioned under materials and methods, and subsequently treated with ECL and exposed to x-ray film. Developed X-ray film was superimposed on the top of the Coomassie stained second gel as well as on the Coomassie stained PVDF filter. Three spots A, C, X were marked, excised and was used for extraction of protein for subsequent Mass spectrometric analyses.
3. The Coomassie stained zebrafish cardiac muscle protein across the gel.
4. Same as in b. However, in this gel 2 spots D & E were marked for subsequent Mass Spectrometric analyses.