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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Cytoskeleton (Hoboken). 2017 Jan 24;74(3):125–142. doi: 10.1002/cm.21352

Figure 2. Conventional RT-PCR amplification of TPM1α and TPM1κ with RNA from heart and skeletal muscle of Zebrafish.

Figure 2

  1. Ethidium bromide staining of the gel
  2. Hybridization with TPM1-1κ specific probe, the sequences of which are in Table 1.
  3. Hybridization withTPM1α specific probe. The nucleotide sequences are given in Table 1
.

Lane 1. Amplification of both TPM1α and TPM1κ by generic primer-pair (Gen TPM1-1α&κ(+)/Gen TPM1-1α&κ(−) as given in Table 1) in heart; lane 2. Amplification of both TPM1α and TPM1κ by generic primer-pair in sk muscle; lane 3. Primer control; lane M. Marker; lane 4. Amplification of TPM1κ by isoform specific primer pair (TPM1-1κ Primer (+)/Gen TPM1-1α&κ(−) as given in Table 1) in heart; lane 5. Amplification of TPM1κ in skeletal muscle; lane 6. Primer control.